Somatic Hybridization
For understanding somatic hybridization you need to understand some basic terms such as
Protoplast :it is a isolated cell which do not have cell wall and bound by
plasmalemma.
Somatic: it is taken forma Acient word Soma which means body.
Hybridization it is a interbreeding process in which new individual is formed by the fusion of two differnet species.
So the definition is new individual body is formed by two differnet species. Or you can say that another definition :
Development of new hybrid are are formed by the fusion of somatic protoplast of two different plant.the protoplast is made to fused using PEG ( polyethylene)or electric fusion.
Procedure: two differnet species of plant cell is taken and by ezymatic action of celluase, the cell wall of plant is break. The protoplast is appeared ,then the protoplast is fused. When we regenerate the fused protoplast , the new hybrid is introduce which take the both superior character of plant species.
Such as pomato in which one potato cell and another one is tomato cell when potato cell fused with tomato cell, the new plant arises which known as pomato. The pomato take both the characteristic of potato and tomato. pomato plant is able to give potato as well as tomato.
Procedure of creating pomato plant at your garden:
It was originally developed in 1930 by Oscar soderholm in worchestor.
Take red cherry tomato and potato plant of similar height as near about 0.1cm or 4 inch then take a sharp knife or sterlie blade and cut the top off both plant gently. And then remove the the middle part of stem from a potato plant and give V shape to it . after this cut the root of tomato plant and then make a simple cut on tomato stem fix the both stem together by grafting tape ensuring that it hold each other by this they share nutrition from each other and start their growth. For creating more chance to grow, placed grafting plant in that area where is not a rich source of Sunlight present, after two or three weeks when the grafting plant shows some growth, remove the grafting tape and you can also transform the plant where u want.
- Somatic hybrid are two types:
- Symmetric hybrid
- Asymmetric hybrid
Cybrid: it is process in which cytoplasm is fused but nucleus is not fused whereas somatic hybridization the cytoplasm as well as nucleus are fused .
application of somatic hybridization:
- We get Disease resistance plant .
- Environment tolerance: genes are able to tolerate the cold, Frost and slat.
- Quality assurance :somatic hybrid for the production of high nicotine content add low euric acid have been developed.
- Cytoplasmic male sterility: modification of hybridization in the form of hybridization has made it possible to transfer male sterility.
- It help in plant breeding program.
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